Bioinformatic Analysis in Epigenetics Research (Nick Pervolarakis)

In this episode of the Epigenetics Podcast, we caught up with Nick Pervolarakis from Active Motif to talk about bioinformatic analysis in epigenetics research.

While many “bench scientists” are familiar with the workflows of ChIP-Seq, ATAC-Seq and CUT&Tag, and even the preparation and analysis of the libraries, the steps between sequencing and fully analyzed data is sometimes thought of as a mystery known only to bioinformatic experts. Most of us have some understanding that the raw data is usually in a file format called a FASTQ. But how do we get from FASTQ files to peaks on a genome browser? This Podcast Episode will provide a peek behind the curtain of the informatic analysis we perform at Active Motif, as part of our end-to-end epigenetic services.

References

• Life in the FASTQ Lane

• Bioinformatics Resource Center

• Epigenetic Services

Related Episodes

• Multiple challenges of ATAC-Seq, Points to Consider (Yuan Xue)

• Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

• Multiple Challenges in ChIP (Adam Blattler)

Contact

• Active Motif on Twitter

• Epigenetics Podcast on Twitter

• Active Motif on LinkedIn

• Active Motif on Facebook

• Email: podcast@activemotif.com

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