289: MinION detection of chimeric reads in murine Ifna/Ifnb amplicons and ligation-related artifact prevalence
Base by Base13 Helmi

289: MinION detection of chimeric reads in murine Ifna/Ifnb amplicons and ligation-related artifact prevalence

White R et al., F1000Research - Investigation of chimeric reads in MinION nanopore sequencing of short PCR amplicons, focusing on ligation-related artifacts, barcode tracing, and the prevalence of cross-gene chimeras in murine Ifna/Ifnb sequencing runs.

Music:
Enjoy the music based on this article at the end of the episode.

Article title:
Investigation of chimeric reads using the MinION

First author:
White R

Journal:
F1000Research

DOI:
10.12688/f1000research.11547.2

Reference:
White R, Pellefigues C, Ronchese F, Lamiable O, Eccles D. Investigation of chimeric reads using the MinION [version 2; peer review: 2 approved]. F1000Research. 2017;6:631. https://doi.org/10.12688/f1000research.11547.2

License:
This episode is based on an open-access article published under the Creative Commons Attribution 4.0 International License (CC BY 4.0) – https://creativecommons.org/licenses/by/4.0/

Support:
Base by Base – Stripe donations: https://donate.stripe.com/7sY4gz71B2sN3RWac5gEg00

Official website https://basebybase.com

On PaperCast Base by Base you’ll discover the latest in genomics, functional genomics, structural genomics, and proteomics.

QC:
This episode was reviewed as a special editorial release.

QC Scope:
- human-authored narration, music, and publication metadata
- excludes automated single-article AI QC because this episode was not produced by the standard AI narration pipeline
- source attribution and descriptive metadata were reviewed manually

QC Summary:
- production mode: human-written and human-produced episode
- automated transcript-vs-paper AI QC: not applicable
- metadata, source attribution, and release assets were reviewed manually

Metadata Audited:
- article_doi
- article_title
- article_journal
- reference
- license

Factual Items Audited:
- episode classified as a human-produced release
- canonical human narration retained as the source of record
- canonical human-produced music retained as the source of record
- automated NotebookLM regeneration intentionally not used for this episode

QC result: Editorial exception. This episode was approved after manual review.

Tämä jakso on lisätty Podme-palveluun avoimen RSS-syötteen kautta eikä se ole Podmen omaa tuotantoa. Siksi jakso saattaa sisältää mainontaa.

Jaksot(462)

461: Adult Ank3 loss quiets neurons and lowers a myelin protein

461: Adult Ank3 loss quiets neurons and lowers a myelin protein

Yoon et al., Proceedings of the National Academy of Sciences - ANK3, which encodes the scaffolding protein ankyrin-G, is a major risk gene for bipolar disorder and schizophrenia, yet what it does in a...

24 Syys 24min

460: The lupus variant that also sharpens antiviral defense

460: The lupus variant that also sharpens antiviral defense

Virolainen et al., The American Journal of Human Genetics - A lupus signal on chromosome 11p15 narrows to a coding haplotype in IRF7 that most people in the world carry. This study shows the risk form...

21 Syys 24min

459: Cerebral palsy genetics: 515 candidate genes, evidence for 89

459: Cerebral palsy genetics: 515 candidate genes, evidence for 89

Arterbery et al., The American Journal of Human Genetics - Hundreds of genes have been reported as causes of cerebral palsy, yet there is no agreed model of what a pathogenic variant in a child with C...

13 Syys 24min

458: Somatic or inherited? Reading TP53 risk from shared DNA

458: Somatic or inherited? Reading TP53 risk from shared DNA

MacGregor et al., The American Journal of Human Genetics - Pathogenic TP53 variants found in blood have long been read as inherited Li-Fraumeni alleles, but many turn out to be somatic clones that gre...

10 Syys 25min

457: A deletion that raises Alzheimer risk, a duplication that lowers it

457: A deletion that raises Alzheimer risk, a duplication that lowers it

Quenez O et al., The American Journal of Human Genetics - Rare copy-number variants were called from 22,319 exomes covering early-onset Alzheimer disease, late-onset disease and unaffected controls, t...

9 Syys 24min

456: Beyond exons: where heritability hides as traits get more polygenic

456: Beyond exons: where heritability hides as traits get more polygenic

Fuhrer J et al., The American Journal of Human Genetics - Across 34 complex traits and disorders, a MiXeR-based framework partitions SNP heritability over 74 functional annotations and finds that exon...

8 Syys 45min

455: Agentic genomics: the bottleneck moves from code to judgment

455: Agentic genomics: the bottleneck moves from code to judgment

Corpas M et al., Cell Genomics - A Perspective arguing that autonomous AI agents which discover, configure and chain bioinformatics operations from natural-language instructions have shifted the bottl...

7 Syys 13min

454: Efavirenz retarda a doença priônica mexendo no colesterol do cérebro [PT]

454: Efavirenz retarda a doença priônica mexendo no colesterol do cérebro [PT]

Ali T et al., JCI Insight - Um antirretroviral aprovado para HIV, dado por via oral em microdose, prolongou a sobrevida de camundongos que carregam a proteína priônica humana e foram infectados com pr...

2 Syys 17min

Suosittua kategoriassa Tiede

rss-mita-tulisi-tietaa
rss-poliisin-mieli
tiedekulma-podcast
utelias-mieli
rss-hereilla
rss-luontopodi-samuel-glassar-tutkii-luonnon-ihmeita
rss-duodecim-lehti
rss-tiedetta-vai-tarinaa
hippokrateen-vastaanotolla
rss-bios-podcast
docemilia
radio-antro
rss-ranskaa-raakana
rss-radplus
rss-lihavuudesta-podcast
rss-politiikasta-podcast