Epigenetics Podcast28 Tammi 2020

PIXUL: On the Leading Edge of Chromatin Shearing (Karol Bomsztyk and Tom Matula)

In this episode of the Epigenetics Podcast, we caught up with Karol Bomsztyk M.D. and Tom Matula, Ph.D. from the University of Washington and Matchstick Technologies, to talk about their work on DNA and chromatin sonication.

During his career, Karol's research has focused on improving ChIP protocols to make them faster, easier and higher throughput. First, to make ChIP assays faster, Karol and his lab developed "Fast-ChIP". More recently, he adjusted this protocol to improve throughput and "Matrix-ChIP" was born. Tom is an expert in the field of ultrasound and cavitation and the Director of the Center for Industrial and Medical Ultrasound at the University of Washington.

To further improve and speed up the 96-well "Matrix-ChIP" protocol, Karol and Tom teamed up to found Matchstick Technologies and develop a sonication device that would be able to processes each and every well of a 96-well microplate consistently and quickly. The result of this cooperation is the PIXUL Multi-Sample Sonicator that is now available for order from Active Motif.

PIXUL is an ultrasound-based sample preparation platform that was designed completely from the ground up to provide researchers with an easy-to-use tool that is simple to set up, simple to use, and generates consistent results day in and day out. No other sample preparation platform out there can match the power and convenience of PIXUL.

PIXUL was conceived by an epigenetics researcher, and designed and built by ultrasound engineers to take the guesswork out of sample preparation. With PIXUL, sample preparation is no longer an art form, but instead a simple and predictable part of experiments that work every single time.

This interview goes into the mechanism behind sonication-based shearing of DNA and chromatin and highlights how PIXUL is different from existing sonication instruments.

References

Contact

Tämä jakso on lisätty Podme-palveluun avoimen RSS-syötteen kautta eikä se ole Podmen omaa tuotantoa. Siksi jakso saattaa sisältää mainontaa.

Jaksot(182)

Chromatin State Reprogramming in Cancer Progression (Kunal Rai)

In this episode of the Epigenetics Podcast, we talked with Kunal Rai from MD Anderson Cancer Center about his work on chromatin state reprogramming in cancer progression. A major part of the convers...

27 Elo 45min

Statistical Physics Approaches to DNA Methylation and Aging (Steffen Rulands)

In this episode of the Epigenetics Podcast, we talked with Steffen Rulands from the Ludwig Maximilian University of Munich about how methods from statistical physics can be used to study collective ph...

13 Elo 54min

From GAL4 to targeted DAM-ID: Tools for Studying Gene Expression In Vivo (Andrea Brand)

In this episode, we speak with Andrea Brand, Chair of the Department of Cell Biology at NYU Grossman School of Medicine and Director of the Regenerative Medicine Institute. We discuss her scientific p...

30 Heinä 44min

Antibodies, Automation, and the Fight for Reproducibility (Alon Goren)

In this episode we speak with Alon Goren from UC San Diego about his work at the intersection of genomic technology development and chromatin biology. We discuss how his lab studies how the epigenome ...

16 Heinä 52min

Long-Term Maintenance of Neuronal Identity (Tomohisa Toda)

In this episode of the Epigenetics Podcast, we talked with Tomohisa Toda from the Max-Planck-Zentrum für Physik und Medizin about his work on the long-term maintenance of neuronal identity, with a foc...

2 Heinä 38min

Enhancer RNAs: Discovery and Function (Tae-Kyung Kim)

In this episode of the Epigenetics Podcast, we talked with Tae-Kyung Kim from POSTECH in South Korea about the discovery and characterisation of enhancer RNAs. Dr. Kim describes joining Danny Reinbe...

18 Kesä 43min

Peter Becker: A Career in Chromatin — From ISWI to Dosage Compensation

In this episode of the Epigenetics Podcast, we talked with Peter Becker from the Biomedical Center Munich about his successful career in Epigenetics, where he discovered the chromatin remodeler ISWI ...

4 Kesä 1h 4min

Transcriptional and Epigenetic Regulation of Craniofacial Development (Filippo Rijli)

In this episode of the Epigenetics Podcast, we talked with Filippo Rijli from the Friedrich Miescher Institute about his work on transcriptional and epigenetic regulation of craniofacial and neuronal ...

21 Touko 51min