H3K4me3, Set-Proteins, Isw1 and their Role in Transcription (Jane Mellor)
Epigenetics Podcast13 Tammi 2022

H3K4me3, Set-Proteins, Isw1 and their Role in Transcription (Jane Mellor)

In this episode of the Epigenetics Podcast, we caught up with Jane Mellor from the University of Oxford to talk about her work on H3K4me3, SET proteins, Isw1 and their role in transcription.

Since the beginning of the century, Jane Mellor and her team have focused on H3K4 trimethylation and the factors that influence this mark. They discovered that H3K4me3 is an almost universal mark of the first nucleosome in every transcribed unit and all organisms. She could subsequently, together with the Kouzarides lab, identify SetD1, the enzyme that is responsible for writing this modification. Later on, the team characterized Isw1, a chromatin remodeler which “reads” H3K4me3. More recently the lab focuses on how the polymerase transcribes throughout the first nucleosomes of the transcribed region at the +2 nucleosome, with the help of Spt4.

References

  • Santos-Rosa, H., Schneider, R., Bannister, A. J., Sherriff, J., Bernstein, B. E., Emre, N. C. T., Schreiber, S. L., Mellor, J., & Kouzarides, T. (2002). Active genes are tri-methylated at K4 of histone H3. Nature, 419(6905), 407–411. https://doi.org/10.1038/nature01080

  • Morillon, A., O’Sullivan, J., Azad, A., Proudfoot, N., & Mellor, J. (2003). Regulation of Elongating RNA Polymerase II by Forkhead Transcription Factors in Yeast. Science, 300(5618), 492–495. https://doi.org/10.1126/science.1081379

  • Morillon, A., Karabetsou, N., O’Sullivan, J., Kent, N., Proudfoot, N., & Mellor, J. (2003). Isw1 Chromatin Remodeling ATPase Coordinates Transcription Elongation and Termination by RNA Polymerase II. Cell, 115(4), 425–435. https://doi.org/10.1016/S0092-8674(03)00880-8

  • Uzun, Ü., Brown, T., Fischl, H., Angel, A., & Mellor, J. (2021). Spt4 facilitates the movement of RNA polymerase II through the +2 nucleosomal barrier. Cell Reports, 36(13), 109755. https://doi.org/10.1016/j.celrep.2021.109755

Related Episodes

Contact

Jaksot(167)

The Interplay of Nutrition, Metabolic Pathways, and Epigenetic Regulation (Ferdinand von Meyenn)

The Interplay of Nutrition, Metabolic Pathways, and Epigenetic Regulation (Ferdinand von Meyenn)

In this episode of the Epigenetics Podcast, we talked with Ferdinand von Meyenn from ETH Zürich about his work on the interplay of nutrition, metabolic pathways, and epigenetic regulation. To start Dr...

23 Tammi 202548min

Single-Molecule Adenine Methylated Oligonucleosome Sequencing Assay (SAMOSA) (Vijay Ramani)

Single-Molecule Adenine Methylated Oligonucleosome Sequencing Assay (SAMOSA) (Vijay Ramani)

In this episode of the Epigenetics Podcast, we talked with Vijay Ramani from the Gladstone Institute about his work on Single-Molecule Adenine Methylated Oligonucleosome Sequencing Assay (SAMOSA). Our...

9 Tammi 202552min

Epigenetic Consequences of DNA Methylation in Development (Maxim Greenberg)

Epigenetic Consequences of DNA Methylation in Development (Maxim Greenberg)

In this episode of the Epigenetics Podcast, we talked with Maxim Greenberg from the Institute Jacob Monot about his work on epigenetic consequences of DNA methylation in development. In this interview...

19 Joulu 202444min

R-Loop Biology in Health and Disease (Natalia Gromak)

R-Loop Biology in Health and Disease (Natalia Gromak)

In this episode of the Epigenetics Podcast, we talked with Natalia Gromak from the University of Oxford about her work on R-Loop biology in health and disease. In this interview Dr. Gromak delves into...

5 Joulu 202429min

The Menin-MLL Complex and Small Molecule Inhibitors (Yadira Soto-Feliciano)

The Menin-MLL Complex and Small Molecule Inhibitors (Yadira Soto-Feliciano)

In this episode of the Epigenetics Podcast, we talked with Yadira Soto-Feliciano from MIT about her work on the Menin-MLL complex and the effect of small molecules on its stability in leukemia. We exp...

21 Marras 202440min

Grant Writing in Academia and Industry – Tips for Success (Mary Anne Jelinek)

Grant Writing in Academia and Industry – Tips for Success (Mary Anne Jelinek)

In this episode of the Epigenetics Podcast, we talked with Mary Anne Jelinek Associate Director of R&D at Active Motif about writing and reviewing grants in academia and industry. Learn from Dr. Jelin...

14 Marras 202439min

DNase Hypersensitive Sites and Chromatin Remodeling Enzymes (Carl Wu)

DNase Hypersensitive Sites and Chromatin Remodeling Enzymes (Carl Wu)

In this episode of the Epigenetics Podcast, we talked with Carl Wu from John's Hopkins University about his work on nucleosome remodeling, histone variants, and the role of single-molecule imaging in ...

31 Loka 202455min

Epigenetic Mechanisms of Mammalian Germ Cell Development (Mitinori Saitou)

Epigenetic Mechanisms of Mammalian Germ Cell Development (Mitinori Saitou)

In this episode of the Epigenetics Podcast, we talked with Mitinori Saitou from Kyoto University about his work on germ cell development, focusing on proteins like BLIMP1 and PRDM14, reprogramming iPS...

17 Loka 202439min

Suosittua kategoriassa Tiede

rss-mita-tulisi-tietaa
rss-poliisin-mieli
rss-lihavuudesta-podcast
utelias-mieli
rss-duodecim-lehti
tiedekulma-podcast
rss-opeklubi
docemilia
mielipaivakirja
hippokrateen-vastaanotolla
radio-antro
rss-mental-race
rss-luontopodi-samuel-glassar-tutkii-luonnon-ihmeita
rss-sosiopodi