NSH Poster Podcast: P007 (2023)

NSH Poster Podcast: P007 (2023)

Gene Protein Assay(GPA): Unveiling Tumor Heterogeneity in HER2/neu Positive Breast Cancer

Authors: Dr. Tanuja Shet, Dr. Aditi Rathi

Introduction: HER2/neu gene amplification on Fluorescence in-situ hybridization (FISH) is defined as average HER2neu gene signals > 4 and HER2:CEP17 ratio of > 2, in breast cancer. However, with tumor heterogeneity interpretation of these cut-offs is challenging and some cases test as borderline. We attempted a study analyzing Gene protein assay (GPA) which combines HER2/neu immunohistochemistry (IHC), and D-DISH (dual color dual in-situ hybridization) assay to help resolve this issue.

Methods: A total of 31 cases reported as HER2/neu amplified with intermixed tumor heterogeneity on FISH were taken for this study. The original HER2/neu count was between 4 to 6 and ratio near 2 in most cases. GPA was done using 4B5 HER2/neu IHC and Roche/Ventana D-DISH kit on Ventana Ultra machine by doing IHC first followed by D-DISH.

Result: On GPA, 3/31 cases were reported as non-amplified, and the rest were amplified. Advantage of GPA was that it helped count D-DISH as per membrane staining and identified more tumor heterogeneity in contrast to FISH in six cases. The results of the remaining cases were the same.

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Jaksot(100)

Fixation on Histology: How Histotechnology Plays a Role in Lung Transplant Technology Advancement

Fixation on Histology: How Histotechnology Plays a Role in Lung Transplant Technology Advancement

Fixation on Histology: How Histotechnology Plays a Role in Lung Transplant Technology Advancement Written by: Napoleon Law, BSc, HTL(ASCP)CM To read the full article, click here.

25 Kesä 8min

Fixation on Histology: A New Approach to Visualizing Blood-Brain Barrier Penetration

Fixation on Histology: A New Approach to Visualizing Blood-Brain Barrier Penetration

Fixation on Histology: A New Approach to Visualizing Blood-Brain Barrier Penetration Written based on an article published in the Journal of Histotechnology To read the full article, click here.

11 Kesä 4min

Fixation on Histology: Understanding Key Differences Between Histology and Mohs

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Fixation on Histology: Understanding Key Differences Between Histology and Mohs To read the full blog, click here.

29 Touko 2min

Fixation on Histology: Two Is Better Than One… At Least When It Comes to Measures of Central Tendency

Fixation on Histology: Two Is Better Than One… At Least When It Comes to Measures of Central Tendency

Fixation on Histology: Two Is Better Than One… At Least When It Comes to Measures of Central Tendency Written by: Connie Wildeman, Director of Education, National Society for Histotechnology Click Her...

15 Touko 4min

Fixation on Histology: Garbage In, Garbage out- The Fixation Rule We Can't Ignore

Fixation on Histology: Garbage In, Garbage out- The Fixation Rule We Can't Ignore

Fixation on Histology: Garbage In, Garbage out- The Fixation Rule We Can't Ignore Written by: NSH Member Napoleon Law, HTL(ASCP)CM To Read the Full Blog, Click Here.

1 Touko 5min

Fixation on Histology: How to Promote Quality Standards Through Effective Communication

Fixation on Histology: How to Promote Quality Standards Through Effective Communication

Fixation on Histology: How to Promote Quality Standards Through Effective Communication To Read the Full Blog, Click Here

17 Huhti 3min

Fixation on Histology: Understanding Multiplex IHC

Fixation on Histology: Understanding Multiplex IHC

Fixation on Histology: Understanding Multiplex IHC To read the full blog, click here.

3 Huhti 3min

Fixation on Histology: A New Approach to Bladder Cancer Detection

Fixation on Histology: A New Approach to Bladder Cancer Detection

Fixation on Histology: A New Approach to Bladder Cancer Detection Written based on the research article published in the Journal of Histotechnology To Read the Full Blog, Click Here.

20 Maalis 4min

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