NSH Poster Podcast: P35 (2024)

NSH Poster Podcast: P35 (2024)

The Development of a Cocktail of Microglia and GFAP For Easy Diagnosis - Anisha Bhasin B.S, Sarah Holguin, MBA. B.S, Joe Vargas, M.S

Microglia and GFAP are distinct neural markers, typically used separately to diagnose the degree of neurological infection and injury. Microglia, a glial cell, is used in the immune response of the central nervous system. GFAP is an astrocyte marker; astrocytes provide structural support and make up the blood-brain barrier. Using the two in conjugation with one another would prove to be an efficient diagnostic tool. A cocktail was constructed with optimal titration to observe the two markers in unison. In clinical usage, it will provide an efficient diagnosis of chronic inflammatory conditions of the central nervous system. The staining was conducted in IHC and fluorescence to compare morphology and count. Due to anatomical similarities, there tends to be morphological confusion between microglia and GFAP. However, when stained in conjunction with one another, notable differences can allow for easy distinction. This is why a cocktail run with a dual staining technique would be a superior diagnostic tool in comparison to testing the two markers independently.

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Jaksot(100)

Fixation on Histology: Thinking About Digital Pathology? Here's What to Look for in a Scanner

Fixation on Histology: Thinking About Digital Pathology? Here's What to Look for in a Scanner

Fixation on Histology: Thinking About Digital Pathology? Here's What to Look for in a Scanner Blog written based on the NSH webinar, The Digital Pathology Journey To Read the Full Blog, Click Here

9 Heinä 3min

Fixation on Histology: How Histotechnology Plays a Role in Lung Transplant Technology Advancement

Fixation on Histology: How Histotechnology Plays a Role in Lung Transplant Technology Advancement

Fixation on Histology: How Histotechnology Plays a Role in Lung Transplant Technology Advancement Written by: Napoleon Law, BSc, HTL(ASCP)CM To read the full article, click here.

25 Kesä 8min

Fixation on Histology: A New Approach to Visualizing Blood-Brain Barrier Penetration

Fixation on Histology: A New Approach to Visualizing Blood-Brain Barrier Penetration

Fixation on Histology: A New Approach to Visualizing Blood-Brain Barrier Penetration Written based on an article published in the Journal of Histotechnology To read the full article, click here.

11 Kesä 4min

Fixation on Histology: Understanding Key Differences Between Histology and Mohs

Fixation on Histology: Understanding Key Differences Between Histology and Mohs

Fixation on Histology: Understanding Key Differences Between Histology and Mohs To read the full blog, click here.

29 Touko 2min

Fixation on Histology: Two Is Better Than One… At Least When It Comes to Measures of Central Tendency

Fixation on Histology: Two Is Better Than One… At Least When It Comes to Measures of Central Tendency

Fixation on Histology: Two Is Better Than One… At Least When It Comes to Measures of Central Tendency Written by: Connie Wildeman, Director of Education, National Society for Histotechnology Click Her...

15 Touko 4min

Fixation on Histology: Garbage In, Garbage out- The Fixation Rule We Can't Ignore

Fixation on Histology: Garbage In, Garbage out- The Fixation Rule We Can't Ignore

Fixation on Histology: Garbage In, Garbage out- The Fixation Rule We Can't Ignore Written by: NSH Member Napoleon Law, HTL(ASCP)CM To Read the Full Blog, Click Here.

1 Touko 5min

Fixation on Histology: How to Promote Quality Standards Through Effective Communication

Fixation on Histology: How to Promote Quality Standards Through Effective Communication

Fixation on Histology: How to Promote Quality Standards Through Effective Communication To Read the Full Blog, Click Here

17 Huhti 3min

Fixation on Histology: Understanding Multiplex IHC

Fixation on Histology: Understanding Multiplex IHC

Fixation on Histology: Understanding Multiplex IHC To read the full blog, click here.

3 Huhti 3min

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