NSH Poster Podcase: P54 (2024)

NSH Poster Podcase: P54 (2024)

Method for FFPE Organ-Chip -Lindsay Parmelee HTL(ASCP) 1,2,3, Stephanie Pei Tung Yiu PhD 1,2,3,4, Chi Ngai Chan PhD 1,2,3, Sizun Jiang PhD 1,2,31 Center for Virology and Vaccine Research 2 Beth Israel Deaconess Medical Center 3 Harvard Medical School 4 Wyss Institute Organoids are currently being developed for applications in biomedicine such as drug development and disease research. With many organoid models emerging on the market there is growing need to develop methods to adapt organoids to the established work flows and assays that are the foundations of modern research. A method was developed for processing, embedding, and sectioning Organ-Chips for FFPE workflows. Organ-Chips were treated with Histogel and trimmed to fit standard sized cassettes. Followed by processing and embedding as usual before proceeding to the microtome. The resulting slides have successfully been stained with hematoxylin and eosin and immunohistochemistry, as well as shown promising results for use on multiplexing platforms. Although the process is laborious and requires continued refinement the slides should be sufficient for highplex proteomics and transcriptomics methods and can be used in addition to FFPE tissues for research into immunology, oncology, and virology.

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Episoder(100)

Fixation on Histology: Thinking About Digital Pathology? Here's What to Look for in a Scanner

Fixation on Histology: Thinking About Digital Pathology? Here's What to Look for in a Scanner

Fixation on Histology: Thinking About Digital Pathology? Here's What to Look for in a Scanner Blog written based on the NSH webinar, The Digital Pathology Journey To Read the Full Blog, Click Here

9 Jul 3min

Fixation on Histology: How Histotechnology Plays a Role in Lung Transplant Technology Advancement

Fixation on Histology: How Histotechnology Plays a Role in Lung Transplant Technology Advancement

Fixation on Histology: How Histotechnology Plays a Role in Lung Transplant Technology Advancement Written by: Napoleon Law, BSc, HTL(ASCP)CM To read the full article, click here.

25 Jun 8min

Fixation on Histology: A New Approach to Visualizing Blood-Brain Barrier Penetration

Fixation on Histology: A New Approach to Visualizing Blood-Brain Barrier Penetration

Fixation on Histology: A New Approach to Visualizing Blood-Brain Barrier Penetration Written based on an article published in the Journal of Histotechnology To read the full article, click here.

11 Jun 4min

Fixation on Histology: Understanding Key Differences Between Histology and Mohs

Fixation on Histology: Understanding Key Differences Between Histology and Mohs

Fixation on Histology: Understanding Key Differences Between Histology and Mohs To read the full blog, click here.

29 Mai 2min

Fixation on Histology: Two Is Better Than One… At Least When It Comes to Measures of Central Tendency

Fixation on Histology: Two Is Better Than One… At Least When It Comes to Measures of Central Tendency

Fixation on Histology: Two Is Better Than One… At Least When It Comes to Measures of Central Tendency Written by: Connie Wildeman, Director of Education, National Society for Histotechnology Click Her...

15 Mai 4min

Fixation on Histology: Garbage In, Garbage out- The Fixation Rule We Can't Ignore

Fixation on Histology: Garbage In, Garbage out- The Fixation Rule We Can't Ignore

Fixation on Histology: Garbage In, Garbage out- The Fixation Rule We Can't Ignore Written by: NSH Member Napoleon Law, HTL(ASCP)CM To Read the Full Blog, Click Here.

1 Mai 5min

Fixation on Histology: How to Promote Quality Standards Through Effective Communication

Fixation on Histology: How to Promote Quality Standards Through Effective Communication

Fixation on Histology: How to Promote Quality Standards Through Effective Communication To Read the Full Blog, Click Here

17 Apr 3min

Fixation on Histology: Understanding Multiplex IHC

Fixation on Histology: Understanding Multiplex IHC

Fixation on Histology: Understanding Multiplex IHC To read the full blog, click here.

3 Apr 3min

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