From Nucleosome Structure to Function (Karolin Luger)

From Nucleosome Structure to Function (Karolin Luger)

In this episode of the Epigenetics Podcast, we caught up with Karolin Luger, Ph.D., from the University of Colorado in Boulder to talk about her work on solving the crystal structure of the nucleosome and on how histone chaperones like FACT act on chromatin.

During her postdoc with Timothy Richmond at the Swiss Federal Institute of Technology in Zürich, Karolin Luger was the first author on an all-time classic paper called "Crystal structure of the nucleosome core particle at 2.8 A resolution" which was published in Nature. This article was published more than 20 years ago now and it has been cited about 9000 times.

After completing her postdoc, she moved to Colorado to set up her own lab where she continued to work on the structure of the nucleosome and the factors that influence their structure. The most recent Nature paper published by her lab investigated how the FACT complex promotes both disassembly and reassembly of nucleosomes during gene transcription, DNA replication, and DNA repair.

In this interview, we discuss the efforts that went into solving the crystal structure of the nucleosome back in 1997, her work on histone chaperones, and her recent work on how FACT keeps nucleosomes intact after gene transcription.

References

  • K. Luger, A. W. Mäder, … T. J. Richmond (1997) Crystal structure of the nucleosome core particle at 2.8 A resolution (Nature) DOI: 10.1038/38444
  • Yang Liu, Keda Zhou, … Karolin Luger (2020) FACT caught in the act of manipulating the nucleosome (Nature) DOI: 10.1038/s41586-019-1820-0

Contact

Avsnitt(167)

Taking ChIP from Yeast to ENCODE to Enable Genome-Wide Regulatory Protein Mapping (Peggy Farnham)

Taking ChIP from Yeast to ENCODE to Enable Genome-Wide Regulatory Protein Mapping (Peggy Farnham)

In this episode of the Epigenetics Podcast, we talked with Peggy Farnham from the Keck School of Medicine at USC about her work on establishing the ChIP Method in mammalian cells. In this episode, we ...

29 Jan 29min

Spatial-Omics and Machine Learning in Muscle Stem Cell Repair (Will Wang)

Spatial-Omics and Machine Learning in Muscle Stem Cell Repair (Will Wang)

In this episode of the Epigenetics Podcast, we talked with Will Wang from Sanford Burnham Prebys about his work on muscle stem cell repair, regeneration, and aging, exploring spatial-omics and machine...

15 Jan 55min

The Future of Protein–DNA Mapping (Mitch Guttman)

The Future of Protein–DNA Mapping (Mitch Guttman)

In this episode of the Epigenetics Podcast, we talked with Mitch Guttman from Caltec about ChIP-DIP (ChIP-Done In Parallel). ChIP-DIP is a newly developed approach for high-resolution protein–DNA inte...

18 Dec 20251h 2min

Chromatin Modifiers and Their Roles in Brain Development (Fides Zenk)

Chromatin Modifiers and Their Roles in Brain Development (Fides Zenk)

In this episode of the Epigenetics Podcast, we talked with Fides Zenk from the École polytechnique fédérale de Lausanne about her work on transgenerational inheritance in Drosophila and brain organoid...

4 Dec 202528min

Region Capture Micro-C and 3D Genome Structure (Anders Sejr Hansen)

Region Capture Micro-C and 3D Genome Structure (Anders Sejr Hansen)

In this episode of the Epigenetics Podcast, we talked with Anders Sejr Hansen from MIT about his work on the impact of 3D genome structures on gene expression, the roles of proteins like CTCF and cohe...

13 Nov 20251h 3min

Reprogramming Cell Identity through Epigenetic Mechanisms (Vincent Pasque)

Reprogramming Cell Identity through Epigenetic Mechanisms (Vincent Pasque)

In this episode of the Epigenetics Podcast, we talked with Vincent Pasque from KU Leuven about his work on the reprogramming of cell identity through epigenetic mechanisms, particularly during early d...

30 Okt 202540min

The Impact of Chromatin Architecture on Alzheimer's and Parkinson's Disease (Ryan Corces)

The Impact of Chromatin Architecture on Alzheimer's and Parkinson's Disease (Ryan Corces)

In this episode of the Epigenetics Podcast, we talked with Ryan Corces from the Gladstone Institutes about his work on the impact of chromatin architecture on Alzheimer's and Parkinson's Disease. The...

16 Okt 202545min

RNA-Mediated Epigenetic Regulation (Mo Motamedi)

RNA-Mediated Epigenetic Regulation (Mo Motamedi)

In this episode of the Epigenetics Podcast, we talked with Mo Motamedi from the Center for Cancer Research at Massachusetts General Hospital about his work on RNA-mediated epigenetic regulation. The I...

2 Okt 202545min

Populärt inom Vetenskap

p3-dystopia
pojkmottagningen
svd-nyhetsartiklar
dumma-manniskor
allt-du-velat-veta
kapitalet-en-podd-om-ekonomi
det-morka-psyket
rss-ufobortom-rimligt-tvivel-2
sexet
rss-vetenskapsradion-2
halsorevolutionen
rss-vetenskapsradion
medicinvetarna
4health-med-anna-sparre
rss-spraket
dumforklarat
paranormalt-med-caroline-giertz
bildningspodden
vetenskapsradion
hacka-livet