Epigenetics Podcast19 Maj 2020

From Nucleosome Structure to Function (Karolin Luger)

In this episode of the Epigenetics Podcast, we caught up with Karolin Luger, Ph.D., from the University of Colorado in Boulder to talk about her work on solving the crystal structure of the nucleosome and on how histone chaperones like FACT act on chromatin.

During her postdoc with Timothy Richmond at the Swiss Federal Institute of Technology in Zürich, Karolin Luger was the first author on an all-time classic paper called "Crystal structure of the nucleosome core particle at 2.8 A resolution" which was published in Nature. This article was published more than 20 years ago now and it has been cited about 9000 times.

After completing her postdoc, she moved to Colorado to set up her own lab where she continued to work on the structure of the nucleosome and the factors that influence their structure. The most recent Nature paper published by her lab investigated how the FACT complex promotes both disassembly and reassembly of nucleosomes during gene transcription, DNA replication, and DNA repair.

In this interview, we discuss the efforts that went into solving the crystal structure of the nucleosome back in 1997, her work on histone chaperones, and her recent work on how FACT keeps nucleosomes intact after gene transcription.

References

K. Luger, A. W. Mäder, … T. J. Richmond (1997) Crystal structure of the nucleosome core particle at 2.8 A resolution (Nature) DOI: 10.1038/38444
Yang Liu, Keda Zhou, … Karolin Luger (2020) FACT caught in the act of manipulating the nucleosome (Nature) DOI: 10.1038/s41586-019-1820-0

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Avsnitt(175)

Transcriptional and Epigenetic Regulation of Craniofacial Development (Filippo Rijli)

In this episode of the Epigenetics Podcast, we talked with Filippo Rijli from the Friedrich Miescher Institute about his work on transcriptional and epigenetic regulation of craniofacial and neuronal ...

21 Maj 51min

Epigenetic Signatures During Aging and Cancer (Alena van Bömmel)

In this episode of the Epigenetics Podcast, we talked with Alena van Bömmel from the Biomedical Center (BMC) in Munich about her work on the development of interpretable epigenetic clocks and statisti...

7 Maj 37min

Heterochromatin Formation by the HUSH complex (Iva Tchasovnikarova)

In this episode of the Epigenetics Podcast, we talked with Iva Tchasovnikarova from the Wellcome Trust Cancer Research UK Gurdon Institute about her work on heterochromatin formation and epigenetic co...

23 Apr 41min

Cryo-EM and the Dynamics of TFIID and PRC2 (Eva Nogales)

In this episode of the Epigenetics Podcast, we talked with Eva Nogales from UC Berkeley about her work on Cryo-EM and the Dynamics of TFIID and PRC2. We explore the origins of Dr. Nogales's fascinat...

9 Apr 51min

RNA modifications and Gene Expression (Michaela Frye)

In this episode of the Epigenetics Podcast, we talked with Michaela Frye from he German Cancer Research Center (DKFZ) in Heidelberg about her work on the role of RNA modifications and RNA binding prot...

26 Mars 45min

Polycomb and Three-Dimensional Genome Organisation (Oliver Bell)

In this episode of the Epigenetics Podcast, we talked with Oliver Bell from the University of Southern California in Los Angeles about his work on chromatin-based regulatory systems that encode cellul...

12 Mars 48min

From Placeholder Nucleosomes to Zygotic Genome Activation (Patrick Murphy)

In this episode of the Epigenetics Podcast, we talked with Patrick Murphy from Cornell University about his work on gene regulation and cellular identity. Dr. Murphy's research focuses on the molecu...

26 Feb 39min

Decoding Cell Fate Through 3D Genome Organization and Chromatin Dynamics (Srinjan Basu)

In this episode of the Epigenetics Podcast, we talked with Srinjan Basu from Imperial College London to talk about his work on how chromatin architecture and epigenetic mechanisms orchestrate developm...

12 Feb 41min