Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

In this episode of the Epigenetics Podcast, we caught up with Active Motif scientists Casidee McDonough from Epigenetic Services and Kyle Tanguay from R&D to talk about technical details of the CUT&Tag protocol and current developments around this method in our R&D Team.

CUT&Tag, which is short for Cleavage Under Targets and Tagmentation, is a molecular biology method that is used to investigate interactions between proteins and DNA and to identify DNA binding sites for their protein of interest. Although CUT&Tag is similar in some ways to ChIP assays, the starting material for CUT&Tag is live, permeabilized cells or isolated cell nuclei, rather than cells or tissue that have been crosslinked with formaldehyde as is the case when performing ChIP. The CUT&Tag method is very sensitive and has been reported to work with as few as 60 cells for some histone modifications. The ability to work with such small numbers of cells is an advantage for researchers working on specific cell types, such as rare neuronal populations, pancreatic islets, or stem cells that are difficult to obtain in large numbers.

In this Episode we discuss the CUT&Tag workflow in detail, talk about the challenges and pitfalls, give guidelines on how to do a good CUT&Tag experiment and offer a glimpse into the future of CUT&Tag product development at Active Motif.

References

Contact

Avsnitt(167)

DNA Replication, Transcription and R-loops (Stephan Hamperl)

DNA Replication, Transcription and R-loops (Stephan Hamperl)

In this episode of the Epigenetics Podcast, we talked with Dr. Stephan Hamperl from the Helmholtz Zentrum Munich about his work on how conflicts between transcription, replication, and R-loop formatio...

13 Juni 202434min

Mutations of Gene Regulatory Elements in Human Disease (Nadav Ahituv)

Mutations of Gene Regulatory Elements in Human Disease (Nadav Ahituv)

In this episode of the Epigenetics Podcast, we talked with Nadav Ahituv from University of California, San Francisco about his work on mutations of gene regulatory elements in human disease. Using mas...

30 Maj 202449min

Using Single-Cell Multiomics to Characterize Human Developmental Hematopoiesis (Ana Cvejic)

Using Single-Cell Multiomics to Characterize Human Developmental Hematopoiesis (Ana Cvejic)

In this episode of the Epigenetics Podcast, we talked with Ana Cvejic from the Biotech Research & Innovation Centre at the University of Copenhagen about her work on using sc-multiomics to characteris...

16 Maj 202437min

The Impact of Sequence Variation on Transcription Factor Binding (Sven Heinz)

The Impact of Sequence Variation on Transcription Factor Binding (Sven Heinz)

In this episode of the Epigenetics Podcast, we talked with Sven Heinz from the University of California in San Diego about his work on the impact of sequence variation on transcription factor binding ...

2 Maj 202440min

Comparing CUT&Tag to ENCODE ChIP-Seq in Alzheimer's Disease Samples (Sarah Marzi)

Comparing CUT&Tag to ENCODE ChIP-Seq in Alzheimer's Disease Samples (Sarah Marzi)

In this episode of the Epigenetics Podcast, we talked with Sarah Marzi from the UK Dementia Research Institute at Imperial College London about her work on epigenetic changes in Alzheimer's Disease, a...

18 Apr 202446min

The Role of Hat1p in Chromatin Assembly (Mark Parthun)

The Role of Hat1p in Chromatin Assembly (Mark Parthun)

In this episode of the Epigenetics Podcast, we talked with Mark Parthun from Ohio State University about his work on the role of Hat1p in chromatin assembly. Mark Parthun shares insights into his pivo...

4 Apr 202447min

The Impact of Paternal Diet on Offspring Metabolism (Upasna Sharma)

The Impact of Paternal Diet on Offspring Metabolism (Upasna Sharma)

In this episode of the Epigenetics Podcast, we talked with Upasna Sharma from UC Santa Cruz about her work on a number of interesting projects on H2A.Z and telomeres, the impact of paternal diet on of...

21 Mars 202436min

H3K36me3, H4K16ac and Cryptic Transcription in Ageing (Weiwei Dang)

H3K36me3, H4K16ac and Cryptic Transcription in Ageing (Weiwei Dang)

In this episode of the Epigenetics Podcast, we talked with Weiwei Dang from Baylor College of Medicine about his work on molecular mechanisms of aging and the role of H3K36me3 and cryptic transcriptio...

7 Mars 202456min

Populärt inom Vetenskap

p3-dystopia
svd-nyhetsartiklar
pojkmottagningen
dumma-manniskor
allt-du-velat-veta
det-morka-psyket
rss-ufobortom-rimligt-tvivel-2
rss-vetenskapsradion
sexet
medicinvetarna
rss-vetenskapsradion-2
halsorevolutionen
kapitalet-en-podd-om-ekonomi
4health-med-anna-sparre
paranormalt-med-caroline-giertz
dumforklarat
rss-spraket
hacka-livet
bildningspodden
vetenskapsradion