Anchor-Based Bisulfite Sequencing (Ben Delatte)

Anchor-Based Bisulfite Sequencing (Ben Delatte)

In this episode of the Epigenetics Podcast, we caught up with Ben Delatte Research Scientist at Active Motif to talk about his work on Anchor Based Bisulfite Sequencing.

Whole Genome Bisulfite Sequencing (WGBS) is the current standard for DNA methylation profiling. However, this approach is costly as it requires sequencing coverage over the entire genome. Here we introduce Anchor-Based Bisulfite Sequencing (ABBS). ABBS captures accurate DNA methylation information in Escherichia coli and mammals, while requiring up to 10 times fewer sequencing reads than WGBS. ABBS interrogates the entire genome and is not restricted to the CpG islands assayed by methods like Reduced Representation Bisulfite Sequencing (RRBS). The ABBS protocol is simple and can be performed in a single day.

References

Related Episodes

Contact

Avsnitt(171)

Chromatin Structure and Dynamics at Ribosomal RNA Genes (Tom Moss)

Chromatin Structure and Dynamics at Ribosomal RNA Genes (Tom Moss)

In this episode of the Epigenetics Podcast, we caught up with Professor Tom Moss from Université Laval in Québec City, Canada to talk about his work on the chromatin structure and dynamics at ribosoma...

24 Mars 202033min

Epigenetic Origins Of Heterogeneity And Disease (Andrew Pospisilik)

Epigenetic Origins Of Heterogeneity And Disease (Andrew Pospisilik)

In this episode of the Epigenetics Podcast, we caught up with Dr. Andrew Pospisilik from the Van Andel Institute in Grand Rapids, Michigan to talk about his work on the epigenetic origins of heterogen...

19 Feb 202033min

PIXUL: On the Leading Edge of Chromatin Shearing (Karol Bomsztyk and Tom Matula)

PIXUL: On the Leading Edge of Chromatin Shearing (Karol Bomsztyk and Tom Matula)

In this episode of the Epigenetics Podcast, we caught up with Karol Bomsztyk M.D. and Tom Matula, Ph.D. from the University of Washington and Matchstick Technologies, to talk about their work on DNA a...

28 Jan 202045min

Influence of Histone Variants on Chromatin Structure and Metabolism (Markus Buschbeck)

Influence of Histone Variants on Chromatin Structure and Metabolism (Markus Buschbeck)

In this episode of the Epigenetics Podcast, we sat down with Marcus Buschbeck, Group Leader at the Josep Carreras Leukaemia Research Institute in Barcelona, to talk about his work on the histone varia...

16 Dec 201931min

Epigenetic Mechanisms of Aging and Longevity (Shelley Berger)

Epigenetic Mechanisms of Aging and Longevity (Shelley Berger)

In this Episode we sat down with Shelley Berger, Keynote Speaker at the "EMBO | EMBL Symposium: Metabolism Meets Epigenetics" to talk about her work on Epigenetic Mechanisms of Aging and Longevity. On...

21 Nov 201938min

Epigenetics & Glioblastoma: New Approaches to Treat Brain Cancer (Lucy Stead)

Epigenetics & Glioblastoma: New Approaches to Treat Brain Cancer (Lucy Stead)

In this Episode of the Epigenetics Podcast our guest Lucy Stead from the University of Leeds provides insight into her work on intratumor heterogeneity in Glioblastoma. In order to tackle this area sh...

15 Okt 201943min

The Past, Present, and Future of Epigenetics (Joe Fernandez, founder of Active Motif)

The Past, Present, and Future of Epigenetics (Joe Fernandez, founder of Active Motif)

Joe Fernandez, the founder of Active Motif, has played a significant role in the evolution of the biotechnology industry. He’s seen where the industry has been, and he has a good idea where it’s going...

5 Sep 201923min

The Interchromatin Network Model (Ana Pombo)

The Interchromatin Network Model (Ana Pombo)

In this Episode of the Epigenetics Podcast our guest Ana Pombo from the Max-Delbrück-Center in Berlin provides insight in her work on the interplay between gene regulation and genome architecture. To ...

12 Aug 201929min

Populärt inom Vetenskap

p3-dystopia
dumma-manniskor
svd-nyhetsartiklar
doden-hjarnan-kemisten
allt-du-velat-veta
rss-ufo-bortom-rimligt-tvivel-2
bildningspodden
det-morka-psyket
rss-vetenskapsradion
kapitalet-en-podd-om-ekonomi
rss-vetenskapsradion-2
sexet
rss-experimentet
medicinvetarna
paranormalt-med-caroline-giertz
har-vi-akt-till-mars-an
dumforklarat
hacka-livet
naturmorgon
halsorevolutionen