Chromatin Analysis using Mass Spectrometry (Axel Imhof)

Chromatin Analysis using Mass Spectrometry (Axel Imhof)

In this episode of the Epigenetics Podcast, we caught up with Axel Imhof from the Ludwig Maximilian University of Munich in Germany to talk about his work on the identification of chromatin associated proteins using mass spectrometry.

As the head of the Proteomics Core Facility Axel Imhof collaborates with research groups around the world. In addition, in his own lab, he focuses on the assembly and composition of chromatin, how environmental metabolites influence epigenetic marks, and how chromatin factors can be used as markers for pathological states.

In this episode we discuss what has changed in the field of mass spectrometry over the years, how Axel Imhof takes advantage of collaborations, how metabolites influence chromatin, and how he is helping to bring epigenetic profiling via mass spectrometry to the clinic.

References

  • Bonaldi, T., Regula, J. T., & Imhof, A. (2003). The Use of Mass Spectrometry for the Analysis of Histone Modifications. In Methods in Enzymology (Vol. 377, pp. 111–130). Elsevier. https://doi.org/10.1016/S0076-6879(03)77006-2

  • Völker-Albert, M. C., Pusch, M. C., Fedisch, A., Schilcher, P., Schmidt, A., & Imhof, A. (2016). A Quantitative Proteomic Analysis of In Vitro Assembled Chromatin. Molecular & Cellular Proteomics, 15(3), 945–959. https://doi.org/10.1074/mcp.M115.053553

  • Scharf, A. N. D., Meier, K., Seitz, V., Kremmer, E., Brehm, A., & Imhof, A. (2009). Monomethylation of Lysine 20 on Histone H4 Facilitates Chromatin Maturation. Molecular and Cellular Biology, 29(1), 57–67. https://doi.org/10.1128/MCB.00989-08

  • Van den Ackerveken, P., Lobbens, A., Turatsinze, J.-V., Solis-Mezarino, V., Völker-Albert, M., Imhof, A., & Herzog, M. (2021). A novel proteomics approach to epigenetic profiling of circulating nucleosomes. Scientific Reports, 11(1), 7256. https://doi.org/10.1038/s41598-021-86630-3

Related Episodes

Contact

Episoder(173)

Epigenetic Mechanisms in Breast Cancer (Luca Magnani)

Epigenetic Mechanisms in Breast Cancer (Luca Magnani)

In this episode of the Epigenetics Podcast, we talked with Luca Magnani from Institute of Cancer Research and UNIMI in Milan about his work on epigenetic mechanisms of drug resistance and cancer cell ...

21 Aug 202537min

How BRD4 and H2BE Influence Neuronal Activity (Erica Korb)

How BRD4 and H2BE Influence Neuronal Activity (Erica Korb)

In this episode of the Epigenetics Podcast, we talked with Erica Korb from the University of Pennsylvania about her work on BRD4 and the histone variant H2BE, which influences synaptic genes and neuro...

7 Aug 202537min

Mapping the Epigenome: From Arabidopsis to the Human Brain (Joseph Ecker)

Mapping the Epigenome: From Arabidopsis to the Human Brain (Joseph Ecker)

In this episode of the Epigenetics Podcast, we talked with Dr. Joseph Ecker from the Salk Institute about his work on high-resolution genome-wide mapping technologies, specifically how the regulation ...

24 Jul 202544min

The Human Cell Atlas (Sarah Teichmann)

The Human Cell Atlas (Sarah Teichmann)

In this episode of the Epigenetics Podcast, we talked with Sarah Teichmann from the University of Cambridge about the Human Cell Atlas. In the Interview we explore Sarah Teichmann's impressive career ...

10 Jul 202546min

The Discovery of Genomic Imprinting (Azim Surani)

The Discovery of Genomic Imprinting (Azim Surani)

In this episode, Professor Asim Surani, shares how his extensive research has significantly advanced the understanding of how the mammalian germline is specified, the mechanisms governing epigenetic r...

19 Jun 202556min

Exploring DNA Methylation and TET Enzymes in Early Development (Petra Hajkova)

Exploring DNA Methylation and TET Enzymes in Early Development (Petra Hajkova)

In this episode of the Epigenetics Podcast, we talked with Petra Hajkova from the MRC Laboratory of Medical Sciences about her work on epigenetics research on mammalian development, highlighting DNA m...

5 Jun 202539min

Epigenetic Regulation and Small Molecule Innovation in AML: Advances in Translational Leukemia Research (Ani Deshpande)

Epigenetic Regulation and Small Molecule Innovation in AML: Advances in Translational Leukemia Research (Ani Deshpande)

In this episode of the Epigenetics Podcast, we talked with Ani Deshpande from Sanford Burnham Prebys about his work on epigenetic regulation and developing small molecules through high throughput scre...

22 Mai 20251h

Beyond Mom: Rethinking Paternal Influence in Epigenetic Inheritance (Raffaele Teperino)

Beyond Mom: Rethinking Paternal Influence in Epigenetic Inheritance (Raffaele Teperino)

In this episode Dr. Raffaele Teperino shares insights from his ongoing research focused on developmental programming, particularly how paternal health before conception influences not only offspring h...

8 Mai 202559min

Populært innen Vitenskap

fastlegen
tingenes-tilstand
jss
forskningno
liberal-halvtime
rekommandert
sinnsyn
rss-paradigmepodden
villmarksliv
rss-nysgjerrige-norge
fjellsportpodden
tidlose-historier
diagnose
kvinnehelsepodden
rss-inn-til-kjernen-med-sunniva-rose
nevropodden
nordnorsk-historie
tomprat-med-gunnar-tjomlid
rss-overskuddsliv
rss-hoyt-lavt-med-ida-tonseth