Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

In this episode of the Epigenetics Podcast, we caught up with Active Motif scientists Casidee McDonough from Epigenetic Services and Kyle Tanguay from R&D to talk about technical details of the CUT&Tag protocol and current developments around this method in our R&D Team.

CUT&Tag, which is short for Cleavage Under Targets and Tagmentation, is a molecular biology method that is used to investigate interactions between proteins and DNA and to identify DNA binding sites for their protein of interest. Although CUT&Tag is similar in some ways to ChIP assays, the starting material for CUT&Tag is live, permeabilized cells or isolated cell nuclei, rather than cells or tissue that have been crosslinked with formaldehyde as is the case when performing ChIP. The CUT&Tag method is very sensitive and has been reported to work with as few as 60 cells for some histone modifications. The ability to work with such small numbers of cells is an advantage for researchers working on specific cell types, such as rare neuronal populations, pancreatic islets, or stem cells that are difficult to obtain in large numbers.

In this Episode we discuss the CUT&Tag workflow in detail, talk about the challenges and pitfalls, give guidelines on how to do a good CUT&Tag experiment and offer a glimpse into the future of CUT&Tag product development at Active Motif.

References

Contact

Episoder(167)

Bioinformatic Analysis in Epigenetics Research (Nick Pervolarakis)

Bioinformatic Analysis in Epigenetics Research (Nick Pervolarakis)

In this episode of the Epigenetics Podcast, we caught up with Nick Pervolarakis from Active Motif to talk about bioinformatic analysis in epigenetics research. While many “bench scientists” are famili...

3 Nov 202238min

Anchor-Based Bisulfite Sequencing (Ben Delatte)

Anchor-Based Bisulfite Sequencing (Ben Delatte)

In this episode of the Epigenetics Podcast, we caught up with Ben Delatte Research Scientist at Active Motif to talk about his work on Anchor Based Bisulfite Sequencing. Whole Genome Bisulfite Sequenc...

20 Okt 202232min

Enhancer Communities in Adipocyte Differentiation (Susanne Mandrup)

Enhancer Communities in Adipocyte Differentiation (Susanne Mandrup)

In this episode of the Epigenetics Podcast, we caught up with Susanne Mandrup from the University of Southern Denmark to talk about her work on the role of enhancer communities in adipocyte differenti...

6 Okt 202227min

Transposable Elements in Gene Regulation and Evolution (Marco Trizzino)

Transposable Elements in Gene Regulation and Evolution (Marco Trizzino)

In this episode of the Epigenetics Podcast, we caught up with Marco Trizzino from Thomas Jefferson University to talk about his work on transposable elements in gene regulation and evolution. Marco T...

22 Sep 202238min

Hydroxymethylation Landscape in Immunecells (Marcela Sjöberg)

Hydroxymethylation Landscape in Immunecells (Marcela Sjöberg)

In this episode of the Epigenetics Podcast, we caught up with Marcela Sjöberg from the Pontificia Universidad Católica de Chile to talk about her work on the hydroxymethylation landscape in immune cel...

8 Sep 202238min

Single Cell Epigenomics in Neuronal Development (Tim Petros)

Single Cell Epigenomics in Neuronal Development (Tim Petros)

In this episode of the Epigenetics Podcast, we caught up with Tim Petros from the Eunice Kennedy Shriver National Institute of Child Health and Human Development at the NIH to talk about his work on S...

25 Aug 202237min

Oncohistones as Drivers of Pediatric Brain Tumors (Nada Jabado)

Oncohistones as Drivers of Pediatric Brain Tumors (Nada Jabado)

In this episode of the Epigenetics Podcast, we caught up with Nada Jabado from McGill University to talk about her work on oncohistones as drivers of Pediatric Brain Tumors. Nada Jabado and her team ...

11 Aug 202239min

Characterization of Epigenetic States in the Oligodendrocyte Lineage (Gonçalo Castelo-Branco)

Characterization of Epigenetic States in the Oligodendrocyte Lineage (Gonçalo Castelo-Branco)

In this episode of the Epigenetics Podcast, we caught up with Goncalo Castelo-Branco from the Karolinska Institute to talk about his work on the characterization of epigenetic states in the Oligodendr...

28 Jul 202242min

Populært innen Vitenskap

fastlegen
tingenes-tilstand
rekommandert
jss
tomprat-med-gunnar-tjomlid
rss-rekommandert
forskningno
sinnsyn
rss-paradigmepodden
villmarksliv
fjellsportpodden
tidlose-historier
dekodet-2
diagnose
grunnstoffene
rss-nysgjerrige-norge
noen-har-snakket-sammen
nevropodden
vett-og-vitenskap-med-gaute-einevoll
rss-hundehuset