Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

In this episode of the Epigenetics Podcast, we caught up with Active Motif scientists Casidee McDonough from Epigenetic Services and Kyle Tanguay from R&D to talk about technical details of the CUT&Tag protocol and current developments around this method in our R&D Team.

CUT&Tag, which is short for Cleavage Under Targets and Tagmentation, is a molecular biology method that is used to investigate interactions between proteins and DNA and to identify DNA binding sites for their protein of interest. Although CUT&Tag is similar in some ways to ChIP assays, the starting material for CUT&Tag is live, permeabilized cells or isolated cell nuclei, rather than cells or tissue that have been crosslinked with formaldehyde as is the case when performing ChIP. The CUT&Tag method is very sensitive and has been reported to work with as few as 60 cells for some histone modifications. The ability to work with such small numbers of cells is an advantage for researchers working on specific cell types, such as rare neuronal populations, pancreatic islets, or stem cells that are difficult to obtain in large numbers.

In this Episode we discuss the CUT&Tag workflow in detail, talk about the challenges and pitfalls, give guidelines on how to do a good CUT&Tag experiment and offer a glimpse into the future of CUT&Tag product development at Active Motif.

References

Contact

Episoder(167)

Unraveling Mechanisms of Chromosome Formation (Job Dekker)

Unraveling Mechanisms of Chromosome Formation (Job Dekker)

In this episode of the Epigenetics Podcast, we caught up with Job Dekker from the University of Massachusetts Medical School to talk about his work on unraveling mechanisms of chromosome formation. In...

7 Jan 202150min

Transcription and Polycomb in Inheritance and Disease (Danny Reinberg)

Transcription and Polycomb in Inheritance and Disease (Danny Reinberg)

In this episode of the Epigenetics Podcast, we caught up with Dr. Danny Reinberg from the New York University School of Medicine to talk about his work on transcription and polycomb in inheritance and...

17 Des 202050min

The Epigenetics of COVID-19

The Epigenetics of COVID-19

In this episode of the Epigenetics Podcast, we caught up with Dr. Sandra Atlante and Dr. Carlo Gaetano from the Instituti Clinici Scientifici Maugeri in Pavia, Italy, to talk about the roles epigeneti...

3 Des 202044min

Epigenetic Reprogramming During Mammalian Development (Wolf Reik)

Epigenetic Reprogramming During Mammalian Development (Wolf Reik)

In this episode of the Epigenetics Podcast, we caught up with Dr. Wolf Reik, Director at the Babraham Institute in Cambridge, UK, to talk about his work on the role of epigenetic factors in cellular r...

24 Nov 202045min

​In vivo Nucleosome Structure and Dynamics (Srinivas Ramachandran)

​In vivo Nucleosome Structure and Dynamics (Srinivas Ramachandran)

In this episode of the Epigenetics Podcast, we caught up with Dr. Srinivas Ramachandran, Assistant Professor at the University of Colorado, Anschutz Medical Campus, to talk about his work on ​in vivo ...

19 Nov 202050min

Pioneer Transcription Factors and Their Influence on Chromatin Structure (Ken Zaret)

Pioneer Transcription Factors and Their Influence on Chromatin Structure (Ken Zaret)

In this episode of the Epigenetics Podcast, we caught up with Dr. Ken Zaret, Professor in the Department of Cell and Developmental Biology at the Perelman School of Medicine, University of Pennsylvani...

5 Nov 202040min

The Role of Small RNAs in Transgenerational Inheritance in C. elegans (Oded Rechavi)

The Role of Small RNAs in Transgenerational Inheritance in C. elegans (Oded Rechavi)

In this episode of the Epigenetics Podcast, we caught up with Dr. Oded Rechavi, Professor at the University of Tel Aviv, to talk about his work on the role of small RNAs in transgenerational inheritan...

22 Okt 202043min

Development of Site-Specific ChIP Technologies (Hodaka Fujii)

Development of Site-Specific ChIP Technologies (Hodaka Fujii)

In this episode of the Epigenetics Podcast, we caught up with Dr. Hodaka Fujii, Professor of Biochemistry and Genome Biology at Hirosaki University Graduate School of Medicine and School of Medicine, ...

1 Okt 202042min

Populært innen Vitenskap

fastlegen
tingenes-tilstand
rekommandert
jss
tomprat-med-gunnar-tjomlid
rss-rekommandert
forskningno
sinnsyn
rss-paradigmepodden
villmarksliv
fjellsportpodden
tidlose-historier
dekodet-2
diagnose
grunnstoffene
rss-nysgjerrige-norge
noen-har-snakket-sammen
nevropodden
vett-og-vitenskap-med-gaute-einevoll
rss-hundehuset