Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

In this episode of the Epigenetics Podcast, we caught up with Active Motif scientists Casidee McDonough from Epigenetic Services and Kyle Tanguay from R&D to talk about technical details of the CUT&Tag protocol and current developments around this method in our R&D Team.

CUT&Tag, which is short for Cleavage Under Targets and Tagmentation, is a molecular biology method that is used to investigate interactions between proteins and DNA and to identify DNA binding sites for their protein of interest. Although CUT&Tag is similar in some ways to ChIP assays, the starting material for CUT&Tag is live, permeabilized cells or isolated cell nuclei, rather than cells or tissue that have been crosslinked with formaldehyde as is the case when performing ChIP. The CUT&Tag method is very sensitive and has been reported to work with as few as 60 cells for some histone modifications. The ability to work with such small numbers of cells is an advantage for researchers working on specific cell types, such as rare neuronal populations, pancreatic islets, or stem cells that are difficult to obtain in large numbers.

In this Episode we discuss the CUT&Tag workflow in detail, talk about the challenges and pitfalls, give guidelines on how to do a good CUT&Tag experiment and offer a glimpse into the future of CUT&Tag product development at Active Motif.

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Episoder(171)

Mapping the Epigenome: From Arabidopsis to the Human Brain (Joseph Ecker)

Mapping the Epigenome: From Arabidopsis to the Human Brain (Joseph Ecker)

In this episode of the Epigenetics Podcast, we talked with Dr. Joseph Ecker from the Salk Institute about his work on high-resolution genome-wide mapping technologies, specifically how the regulation ...

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The Human Cell Atlas (Sarah Teichmann)

The Human Cell Atlas (Sarah Teichmann)

In this episode of the Epigenetics Podcast, we talked with Sarah Teichmann from the University of Cambridge about the Human Cell Atlas. In the Interview we explore Sarah Teichmann's impressive career ...

10 Jul 202546min

The Discovery of Genomic Imprinting (Azim Surani)

The Discovery of Genomic Imprinting (Azim Surani)

In this episode, Professor Asim Surani, shares how his extensive research has significantly advanced the understanding of how the mammalian germline is specified, the mechanisms governing epigenetic r...

19 Jun 202556min

Exploring DNA Methylation and TET Enzymes in Early Development (Petra Hajkova)

Exploring DNA Methylation and TET Enzymes in Early Development (Petra Hajkova)

In this episode of the Epigenetics Podcast, we talked with Petra Hajkova from the MRC Laboratory of Medical Sciences about her work on epigenetics research on mammalian development, highlighting DNA m...

5 Jun 202539min

Epigenetic Regulation and Small Molecule Innovation in AML: Advances in Translational Leukemia Research (Ani Deshpande)

Epigenetic Regulation and Small Molecule Innovation in AML: Advances in Translational Leukemia Research (Ani Deshpande)

In this episode of the Epigenetics Podcast, we talked with Ani Deshpande from Sanford Burnham Prebys about his work on epigenetic regulation and developing small molecules through high throughput scre...

22 Mai 20251h

Beyond Mom: Rethinking Paternal Influence in Epigenetic Inheritance (Raffaele Teperino)

Beyond Mom: Rethinking Paternal Influence in Epigenetic Inheritance (Raffaele Teperino)

In this episode Dr. Raffaele Teperino shares insights from his ongoing research focused on developmental programming, particularly how paternal health before conception influences not only offspring h...

8 Mai 202559min

Evolutionary Epigenetic Clocks and Epigenetic Inheritance in Plants (Frank Johannes)

Evolutionary Epigenetic Clocks and Epigenetic Inheritance in Plants (Frank Johannes)

In this episode of the Epigenetics Podcast, we talked with Dr. Frank Johannes from the Technical University of Munich in Freising about his work on evolutionary clocks and epigenetic inheritance in pl...

24 Apr 202539min

Neuroepigenetic Mechanisms and Primate Epigenome Evolution (Boyan Bonev)

Neuroepigenetic Mechanisms and Primate Epigenome Evolution (Boyan Bonev)

In this episode of the Epigenetics Podcast, we talked with Boyan Bonev from the HelmholtzZetrum in Munich about his work on neuroepigenetics, focusing on gene regulation, chromatin architecture, and p...

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