Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

In this episode of the Epigenetics Podcast, we caught up with Active Motif scientists Casidee McDonough from Epigenetic Services and Kyle Tanguay from R&D to talk about technical details of the CUT&Tag protocol and current developments around this method in our R&D Team.

CUT&Tag, which is short for Cleavage Under Targets and Tagmentation, is a molecular biology method that is used to investigate interactions between proteins and DNA and to identify DNA binding sites for their protein of interest. Although CUT&Tag is similar in some ways to ChIP assays, the starting material for CUT&Tag is live, permeabilized cells or isolated cell nuclei, rather than cells or tissue that have been crosslinked with formaldehyde as is the case when performing ChIP. The CUT&Tag method is very sensitive and has been reported to work with as few as 60 cells for some histone modifications. The ability to work with such small numbers of cells is an advantage for researchers working on specific cell types, such as rare neuronal populations, pancreatic islets, or stem cells that are difficult to obtain in large numbers.

In this Episode we discuss the CUT&Tag workflow in detail, talk about the challenges and pitfalls, give guidelines on how to do a good CUT&Tag experiment and offer a glimpse into the future of CUT&Tag product development at Active Motif.

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Episoder(167)

Split-Pool Recognition of Interactions by Tag Extension (SPRITE) (Mitch Guttman)

Split-Pool Recognition of Interactions by Tag Extension (SPRITE) (Mitch Guttman)

In this episode of the Epigenetics Podcast, we talked with Mitch Guttman from California Institute of Technology about his work on characterising the 3D interactions of the genome using Split-Pool Rec...

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MLL Proteins in Mixed-Lineage Leukemia (Yali Dou)

MLL Proteins in Mixed-Lineage Leukemia (Yali Dou)

In this episode of the Epigenetics Podcast, we talked with Yali Dou from Keck School of Medicine of USC about her work on MLL Proteins in Mixed-Lineage Leukemia. To start off this Interview Yali descr...

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Sex-biased Imprinting and DNA Regulatory Landscapes During Reprogramming (Sam Buckberry)

Sex-biased Imprinting and DNA Regulatory Landscapes During Reprogramming (Sam Buckberry)

In this episode of the Epigenetics Podcast, we talked with Sam Buckberry from the Telethon Kids Institute about his work on gene imprinting, sex-biased gene expression, DNA regulatory landscapes, and ...

25 Jan 202438min

BET Proteins and Their Role in Chromosome Folding and Compartmentalization (Kyle Eagen)

BET Proteins and Their Role in Chromosome Folding and Compartmentalization (Kyle Eagen)

In this episode of the Epigenetics Podcast, we talked with Kyle Eagen from Baylor College of Medicine about his work on BET Proteins and their role in chromosome folding and compartmentalization. In t...

11 Jan 202430min

Epigenetic Underpinnings of Human Addiction (Francesca Telese & Jessica Zhou)

Epigenetic Underpinnings of Human Addiction (Francesca Telese & Jessica Zhou)

In this episode of the Epigenetics Podcast, we talked with Francesca Telese from UC San Diego and Jessica Zhou from Cold Spring Harbour about their work on the molecular underpinnings of human addicti...

21 Des 202359min

H3K79 Methylation, DOT1L, and FOXG1 in Neural Development (Tanja Vogel)

H3K79 Methylation, DOT1L, and FOXG1 in Neural Development (Tanja Vogel)

In this episode of the Epigenetics Podcast, we talked with Tanja Vogel from the University Clinics Freiburg about her work on epigenetic modifications in stem cells during central nervous system devel...

30 Nov 202342min

Function of Insulators in 3D Genome Folding (Maria Gambetta)

Function of Insulators in 3D Genome Folding (Maria Gambetta)

In this episode of the Epigenetics Podcast, we talked with Maria Gambetta from the University of Lausanne about her work on the function of insulators in 3D genome folding. Maria Gambetta focuses on i...

16 Nov 202343min

Contribution of the Estrogen Receptor to Breast Cancer Progression (Jason Carroll)

Contribution of the Estrogen Receptor to Breast Cancer Progression (Jason Carroll)

In this episode of the Epigenetics Podcast, we talked with Jason Carroll from the Cambridge Research Institute about his work on contribution of estrogen receptor to breast cancer progression. The Pod...

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