Anchor-Based Bisulfite Sequencing (Ben Delatte)

Anchor-Based Bisulfite Sequencing (Ben Delatte)

In this episode of the Epigenetics Podcast, we caught up with Ben Delatte Research Scientist at Active Motif to talk about his work on Anchor Based Bisulfite Sequencing.

Whole Genome Bisulfite Sequencing (WGBS) is the current standard for DNA methylation profiling. However, this approach is costly as it requires sequencing coverage over the entire genome. Here we introduce Anchor-Based Bisulfite Sequencing (ABBS). ABBS captures accurate DNA methylation information in Escherichia coli and mammals, while requiring up to 10 times fewer sequencing reads than WGBS. ABBS interrogates the entire genome and is not restricted to the CpG islands assayed by methods like Reduced Representation Bisulfite Sequencing (RRBS). The ABBS protocol is simple and can be performed in a single day.

References

Related Episodes

Contact

Episoder(167)

The Epigenetics of Human Sperm Cells (Sarah Kimmins)

The Epigenetics of Human Sperm Cells (Sarah Kimmins)

In this episode of the Epigenetics Podcast, we caught up with Sarah Kimmins from Université de Montreal to talk about her work on the epigenetics of human sperm cells. The focus of Sarah Kimmins and h...

9 Mar 202344min

Transgenerational Inheritance and Evolution of Epimutations (Peter Sarkies)

Transgenerational Inheritance and Evolution of Epimutations (Peter Sarkies)

In this episode of the Epigenetics Podcast, we caught up with Peter Sarkies from University of Oxford Biochemistry to talk about his work on Transgenerational Inheritance of Epimutations. The team in ...

23 Feb 202353min

Transcription Elongation Control by the Paf1 Complex (Karen Arndt)

Transcription Elongation Control by the Paf1 Complex (Karen Arndt)

In this episode of the Epigenetics Podcast, we caught up with Karen Arndt from the University of Pittsburgh to talk about her work on transcription elongation control by the Paf1 complex. Karen Arndt ...

9 Feb 202339min

Molecular Mechanisms of Chromatin Modifying Enzymes (Karim-Jean Armache)

Molecular Mechanisms of Chromatin Modifying Enzymes (Karim-Jean Armache)

In this episode of the Epigenetics Podcast, we caught up with Karim-Jean Armache from New York University - Grossman School of Medicine to talk about his work on the structural analysis of Polycomb Co...

26 Jan 202334min

The Role of PHF13 in Chromatin and Transcription (Sarah Kinkley)

The Role of PHF13 in Chromatin and Transcription (Sarah Kinkley)

In this episode of the Epigenetics Podcast, we caught up with Sarah Kinkley from the Max Planck Institute of Molecular Genetics to talk about her work on PHF13 and its role in chromatin and transcript...

12 Jan 202334min

Genome Organization Mediated by RNA Polymerase II (Argyrys Papantonis)

Genome Organization Mediated by RNA Polymerase II (Argyrys Papantonis)

In this episode of the Epigenetics Podcast, we caught up with Akis Papantonis from the University Medical Center Göttingen to talk about his work on genome organisation mediated by RNA Polymerase II. ...

15 Des 202243min

The Role of Pioneer Factors Zelda and Grainyhead at the Maternal-to-Zygotic Transition (Melissa Harrison)

The Role of Pioneer Factors Zelda and Grainyhead at the Maternal-to-Zygotic Transition (Melissa Harrison)

In this episode of the Epigenetics Podcast, we caught up with Melissa Harrison from the University of Wisconsin-Madison to talk about her work on the “Pioneer” Transcription Factors - Zelda and Grainy...

1 Des 202241min

Epigenetics in Human Malaria Parasites (Elena Gómez-Diaz)

Epigenetics in Human Malaria Parasites (Elena Gómez-Diaz)

In this episode of the Epigenetics Podcast, we caught up with Elena Gomez-Diaz from the Institute of Parasitology and Biomedicine López-Neyra at the Spanish National Research Council. She share with u...

17 Nov 202237min

Populært innen Vitenskap

fastlegen
tingenes-tilstand
jss
rekommandert
rss-rekommandert
tomprat-med-gunnar-tjomlid
forskningno
sinnsyn
villmarksliv
fjellsportpodden
rss-paradigmepodden
dekodet-2
tidlose-historier
grunnstoffene
diagnose
vett-og-vitenskap-med-gaute-einevoll
noen-har-snakket-sammen
rss-overskuddsliv
rss-nysgjerrige-norge
rss-skogkurs-podden