Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

In this episode of the Epigenetics Podcast, we caught up with Active Motif scientists Casidee McDonough from Epigenetic Services and Kyle Tanguay from R&D to talk about technical details of the CUT&Tag protocol and current developments around this method in our R&D Team.

CUT&Tag, which is short for Cleavage Under Targets and Tagmentation, is a molecular biology method that is used to investigate interactions between proteins and DNA and to identify DNA binding sites for their protein of interest. Although CUT&Tag is similar in some ways to ChIP assays, the starting material for CUT&Tag is live, permeabilized cells or isolated cell nuclei, rather than cells or tissue that have been crosslinked with formaldehyde as is the case when performing ChIP. The CUT&Tag method is very sensitive and has been reported to work with as few as 60 cells for some histone modifications. The ability to work with such small numbers of cells is an advantage for researchers working on specific cell types, such as rare neuronal populations, pancreatic islets, or stem cells that are difficult to obtain in large numbers.

In this Episode we discuss the CUT&Tag workflow in detail, talk about the challenges and pitfalls, give guidelines on how to do a good CUT&Tag experiment and offer a glimpse into the future of CUT&Tag product development at Active Motif.

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Episoder(171)

Enhancer-Promoter Interactions During Development (Yad Ghavi-Helm)

Enhancer-Promoter Interactions During Development (Yad Ghavi-Helm)

In this episode of the Epigenetics Podcast, we caught up with Yad Ghavi-Helm from the Institut de Génomique Functionnelle de Lyon to talk about her work on enhancer-promoter interactions during develo...

10 Feb 202228min

The Effect of Vitamin D on the Epigenome (Folami Ideraabdullah)

The Effect of Vitamin D on the Epigenome (Folami Ideraabdullah)

In this episode of the Epigenetics Podcast, we caught up with Folami Ideraabdullah from the University of Chapel Hill to talk about her work on the environmental modulation of the epigenome during dev...

27 Jan 202236min

H3K4me3, Set-Proteins, Isw1 and their Role in Transcription (Jane Mellor)

H3K4me3, Set-Proteins, Isw1 and their Role in Transcription (Jane Mellor)

In this episode of the Epigenetics Podcast, we caught up with Jane Mellor from the University of Oxford to talk about her work on H3K4me3, SET proteins, Isw1 and their role in transcription. Since the...

13 Jan 202242min

The role of DNA Methylation in Epilepsy (Katja Kobow)

The role of DNA Methylation in Epilepsy (Katja Kobow)

In this episode of the Epigenetics Podcast, we caught up with Katja Kobow from the Universitätsklinikum Erlangen to talk about her work on the role of DNA methylation in Epilepsy. Katja Kobow started ...

16 Des 202135min

MacroH2A Function in Development and Disease (Emily Bernstein)

MacroH2A Function in Development and Disease (Emily Bernstein)

In this episode of the Epigenetics Podcast, we caught up with Emily Bernstein from Icahn Schoon of Medicine at Mount Sinai to talk about her work on MacroH2A function and the role of Polycomb proteins...

2 Des 202132min

Spatio-Temporal Alterations in Chromosome Dynamics (Jane Skok)

Spatio-Temporal Alterations in Chromosome Dynamics (Jane Skok)

In this episode of the Epigenetics Podcast, we caught up with Jane Skok from New York University School of Medicine to talk about her work on spatio-temporal alterations in chromosome dynamics. Studie...

18 Nov 202142min

Chromatin Organization During Development and Disease (Marieke Oudelaar)

Chromatin Organization During Development and Disease (Marieke Oudelaar)

In this episode of the Epigenetics Podcast, we caught up with Marieke Oudelaar from the Max Planck Institute for Biophysical Chemistry to talk about her work on chromatin organization during developme...

11 Nov 202138min

Enhancers and Chromatin Remodeling in Mammary Gland Development (Camila dos Santos)

Enhancers and Chromatin Remodeling in Mammary Gland Development (Camila dos Santos)

In this episode of the Epigenetics Podcast, we caught up with Camila dos Santos from Cold Spring Harbor Laboratories to talk about her work on enhancers and chromatin remodeling in mammary gland devel...

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