Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

In this episode of the Epigenetics Podcast, we caught up with Active Motif scientists Casidee McDonough from Epigenetic Services and Kyle Tanguay from R&D to talk about technical details of the CUT&Tag protocol and current developments around this method in our R&D Team.

CUT&Tag, which is short for Cleavage Under Targets and Tagmentation, is a molecular biology method that is used to investigate interactions between proteins and DNA and to identify DNA binding sites for their protein of interest. Although CUT&Tag is similar in some ways to ChIP assays, the starting material for CUT&Tag is live, permeabilized cells or isolated cell nuclei, rather than cells or tissue that have been crosslinked with formaldehyde as is the case when performing ChIP. The CUT&Tag method is very sensitive and has been reported to work with as few as 60 cells for some histone modifications. The ability to work with such small numbers of cells is an advantage for researchers working on specific cell types, such as rare neuronal populations, pancreatic islets, or stem cells that are difficult to obtain in large numbers.

In this Episode we discuss the CUT&Tag workflow in detail, talk about the challenges and pitfalls, give guidelines on how to do a good CUT&Tag experiment and offer a glimpse into the future of CUT&Tag product development at Active Motif.

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Episoder(167)

Targeting COMPASS to Cure Childhood Leukemia (Ali Shilatifard)

Targeting COMPASS to Cure Childhood Leukemia (Ali Shilatifard)

In this episode of the Epigenetics Podcast, we caught up with Ali Shilatifard from Northwestern University to talk about his work on targeting COMPASS to cure childhood leukemia. The Shilatifard Lab ...

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Effects of Non-Enzymatic Covalent Histone Modifications on Chromatin (Yael David)

Effects of Non-Enzymatic Covalent Histone Modifications on Chromatin (Yael David)

In this episode of the Epigenetics Podcast, we caught up with Yael David from Memorial Sloan Kettering Cancer Center in New York to talk about her work on Effects of Non-Enzymatic Covalent Histone Mod...

5 Aug 202134min

ATAC-Seq, scATAC-Seq and Chromatin Dynamics in Single-Cells (Jason Buenrostro)

ATAC-Seq, scATAC-Seq and Chromatin Dynamics in Single-Cells (Jason Buenrostro)

In this episode of the Epigenetics Podcast, we caught up with Jason Buenrostro from Harvard University to talk about his work on developing biological tools to measure chromatin dynamics in single-cel...

22 Jul 202147min

Synthetic Chromatin Epigenetics (Karmella Haynes)

Synthetic Chromatin Epigenetics (Karmella Haynes)

In this episode of the Epigenetics Podcast, we caught up with Karmella Haynes from Emory University to talk about her work on synthetic chromatin epigenetics. The Haynes lab focuses on the design of ...

8 Jul 202144min

Development of Integrative Machine Learning Tools for Neurodegenerative Diseases (Enrico Glaab)

Development of Integrative Machine Learning Tools for Neurodegenerative Diseases (Enrico Glaab)

In this episode of the Epigenetics Podcast, we caught up with Enrico Glaab from the University of Luxemburg to talk about his work on the development of integrative machine learning tools for neurodeg...

24 Jun 202134min

Ultraconserved Enhancers and Enhancer Redundancy (Diane Dickel)

Ultraconserved Enhancers and Enhancer Redundancy (Diane Dickel)

In this episode of the Epigenetics Podcast, we caught up with Diane Dickel from Lawrence Berkeley National Laboratory to talk about her work on ultraconserved enhancers and enhancer redundancy. Diane ...

10 Jun 202147min

Variants of Core Histones: Modulators of Chromatin Structure and Function (Sandra Hake)

Variants of Core Histones: Modulators of Chromatin Structure and Function (Sandra Hake)

In this episode of the Epigenetics Podcast, we caught up with Sandra Hake from the Justus Liebig University in Giessen to talk about her work on variants of core histones and their role as modulators ...

27 Mai 202133min

DNA Methylation and Mammalian Development (Déborah Bourc'his)

DNA Methylation and Mammalian Development (Déborah Bourc'his)

In this episode of the Epigenetics Podcast, we caught up with Déborah Bourc'his from L'Institut Curie in Paris to talk about her work on the role of DNA methylation in mammalian development. During he...

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