Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

In this episode of the Epigenetics Podcast, we caught up with Active Motif scientists Casidee McDonough from Epigenetic Services and Kyle Tanguay from R&D to talk about technical details of the CUT&Tag protocol and current developments around this method in our R&D Team.

CUT&Tag, which is short for Cleavage Under Targets and Tagmentation, is a molecular biology method that is used to investigate interactions between proteins and DNA and to identify DNA binding sites for their protein of interest. Although CUT&Tag is similar in some ways to ChIP assays, the starting material for CUT&Tag is live, permeabilized cells or isolated cell nuclei, rather than cells or tissue that have been crosslinked with formaldehyde as is the case when performing ChIP. The CUT&Tag method is very sensitive and has been reported to work with as few as 60 cells for some histone modifications. The ability to work with such small numbers of cells is an advantage for researchers working on specific cell types, such as rare neuronal populations, pancreatic islets, or stem cells that are difficult to obtain in large numbers.

In this Episode we discuss the CUT&Tag workflow in detail, talk about the challenges and pitfalls, give guidelines on how to do a good CUT&Tag experiment and offer a glimpse into the future of CUT&Tag product development at Active Motif.

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Episoder(171)

How to Publish in Nature: Lessons from the ENCODE Consortium (Michelle Trenkmann, Senior Editor at Nature)

How to Publish in Nature: Lessons from the ENCODE Consortium (Michelle Trenkmann, Senior Editor at Nature)

In this episode of the Epigenetics Podcast, we caught up with Dr. Michelle Trenkmann, Senior Editor at Nature. We discussed her work as an editor at Nature and how she contributed to the ENCODE 3 publ...

6 Aug 202035min

The Role of Non-Histone Proteins in Chromosome Structure and Function During Mitosis (Bill Earnshaw)

The Role of Non-Histone Proteins in Chromosome Structure and Function During Mitosis (Bill Earnshaw)

In this episode of the Epigenetics Podcast, we caught up with Professor Bill Earnshaw, Wellcome Trust Principal Research Fellow at the University of Edinburgh, to talk about his work on the role of no...

23 Jul 20201h 4min

Effects of DNA Methylation on Chromatin Structure and Transcription (Dirk Schübeler)

Effects of DNA Methylation on Chromatin Structure and Transcription (Dirk Schübeler)

In this episode of the Epigenetics Podcast, we caught up with Dr. Dirk Schübeler, Director of the Friedrich Miescher Institute (FMI) in Basel, Switzerland, to talk about his work on the effects of DNA...

2 Jul 202036min

CpG Islands, DNA Methylation, and Disease (Adrian Bird)

CpG Islands, DNA Methylation, and Disease (Adrian Bird)

In this episode of the Epigenetics Podcast, we caught up with Sir Adrian Bird, Buchanan Professor of Genetics at the University of Edinburgh to talk about his work on CpG islands, DNA methylation, and...

18 Jun 202047min

Biophysical Modeling of 3-D Genome Organization (Leonid Mirny)

Biophysical Modeling of 3-D Genome Organization (Leonid Mirny)

In this episode of the Epigenetics Podcast, we caught up with Leonid Mirny, Ph.D., from MIT to talk about his work on biophysical modeling of the 3-D structure of chromatin. Leonid Mirny was part of t...

4 Jun 202041min

From Nucleosome Structure to Function (Karolin Luger)

From Nucleosome Structure to Function (Karolin Luger)

In this episode of the Epigenetics Podcast, we caught up with Karolin Luger, Ph.D., from the University of Colorado in Boulder to talk about her work on solving the crystal structure of the nucleosome...

19 Mai 202036min

Identification of Functional Elements in the Genome (Bing Ren)

Identification of Functional Elements in the Genome (Bing Ren)

In this episode of the Epigenetics Podcast, we caught up with Bing Ren, Ph.D., from the University of California, San Diego and the Ludwig Institute for Cancer Research to talk about his work on ident...

7 Mai 202043min

Hi-C and Three-Dimensional Genome Sequencing (Erez Lieberman Aiden)

Hi-C and Three-Dimensional Genome Sequencing (Erez Lieberman Aiden)

In this episode of the Epigenetics Podcast, we caught up with Erez Lieberman Aiden, Ph.D. from Baylor College of Medicine and Rice University in Houston to talk about his work on developing Hi-C and i...

23 Apr 202047min

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