Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)

In this episode of the Epigenetics Podcast, we caught up with Active Motif scientists Casidee McDonough from Epigenetic Services and Kyle Tanguay from R&D to talk about technical details of the CUT&Tag protocol and current developments around this method in our R&D Team.

CUT&Tag, which is short for Cleavage Under Targets and Tagmentation, is a molecular biology method that is used to investigate interactions between proteins and DNA and to identify DNA binding sites for their protein of interest. Although CUT&Tag is similar in some ways to ChIP assays, the starting material for CUT&Tag is live, permeabilized cells or isolated cell nuclei, rather than cells or tissue that have been crosslinked with formaldehyde as is the case when performing ChIP. The CUT&Tag method is very sensitive and has been reported to work with as few as 60 cells for some histone modifications. The ability to work with such small numbers of cells is an advantage for researchers working on specific cell types, such as rare neuronal populations, pancreatic islets, or stem cells that are difficult to obtain in large numbers.

In this Episode we discuss the CUT&Tag workflow in detail, talk about the challenges and pitfalls, give guidelines on how to do a good CUT&Tag experiment and offer a glimpse into the future of CUT&Tag product development at Active Motif.

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Episoder(167)

Epigenetic Mechanisms of Aging and Longevity (Shelley Berger)

Epigenetic Mechanisms of Aging and Longevity (Shelley Berger)

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21 Nov 201938min

Epigenetics & Glioblastoma: New Approaches to Treat Brain Cancer (Lucy Stead)

Epigenetics & Glioblastoma: New Approaches to Treat Brain Cancer (Lucy Stead)

In this Episode of the Epigenetics Podcast our guest Lucy Stead from the University of Leeds provides insight into her work on intratumor heterogeneity in Glioblastoma. In order to tackle this area sh...

15 Okt 201943min

The Past, Present, and Future of Epigenetics (Joe Fernandez, founder of Active Motif)

The Past, Present, and Future of Epigenetics (Joe Fernandez, founder of Active Motif)

Joe Fernandez, the founder of Active Motif, has played a significant role in the evolution of the biotechnology industry. He’s seen where the industry has been, and he has a good idea where it’s going...

5 Sep 201923min

The Interchromatin Network Model (Ana Pombo)

The Interchromatin Network Model (Ana Pombo)

In this Episode of the Epigenetics Podcast our guest Ana Pombo from the Max-Delbrück-Center in Berlin provides insight in her work on the interplay between gene regulation and genome architecture. To ...

12 Aug 201929min

Dosage Compensation in Drosophila (Asifa Akhtar)

Dosage Compensation in Drosophila (Asifa Akhtar)

Dosage compensation is an essential process to regulate the gene expression of the X-chromosome in female and male flies. Thereby the mechanism of regulation in humans and in drosophila is different. ...

16 Jul 201936min

Spatial Organization of the Human Genome (Wendy Bickmore)

Spatial Organization of the Human Genome (Wendy Bickmore)

In recent years it has become more and more evident, that genome folding and chromatin packaging into the nucleus plays a pivotal role in the regulation of gene expression. In this Episode of our Podc...

11 Jun 201931min

Heterochromatin and Phase Separation (Gary Karpen)

Heterochromatin and Phase Separation (Gary Karpen)

Heterochromatin plays a pivotal role in organizing our genome in the nucleus and separating active from inactive genomic regions. In this Podcast Episode our Guest Gary Karpen from UC Berkeley sits do...

9 Mai 201934min

Diabetes and Epigenetics (Jean-Sébastien Annicotte)

Diabetes and Epigenetics (Jean-Sébastien Annicotte)

Type 2 Diabetes (T2D) is a chronic metabolic disease, which is caused by the failure of beta-cells in the pancreas and insulin resistance in peripheral tissue and characterized by high glucose levels ...

4 Apr 201918min

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